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Here, we report that chromatin compaction protects genomicDNA from radiation damage.
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Plaque samples were analyzed for 13 subgingival species using whole- genomicDNA probes.
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Such editing involves site-specific digestion of mutant genomicDNA followed by repair.
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Venous blood was collected from family members and genomicDNA was extracted.
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GenomicDNA was extracted from peripheral blood using the 'salting out' procedure.
Usage of gdna in anglès
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Consequently, mutation screening of the IDS gene cannot be restricted to gDNA examination.
2
Mutations were identified by the study of both cDNA and gDNA.
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The same 31 variants were found in the exact same pattern of samples in the gDNA group.
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Results: Altered melting curves was present in 85 of wgaDNA samples and 81 of gDNA samples.
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Methods: cDNA libraries gDNA library was screened with a partial cDNA clone which is homologous to human protein kinase DYRK2 as probe.
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Highly purified gDNA circles are treated with CRISPR-Cas9 ribonucleoprotein complexes, and nuclease-linearized DNA fragments are then ligated to adapters for high-throughput sequencing.
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Conclusion: The SLSO method was found to be inexpensive, non-hazardous and a suitable technique for isolating gDNA from DBS samples for genetic studies.
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These results indicate that SS using BM gDNA and PB cDNA is a rapid and useful method for screening for GATA1 mutations in ML-DS patients.
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Methods: gDNA was extracted from whole blood obtained from 10 patients with lone atrial fibrillation (mean age 22.3 years).